Journal: Scientific Reports

Article Title: Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors

doi: 10.1038/srep14213

Figure Lengend Snippet: Data were normalized and the scale is indicated below the figures. ( a ) Unsupervised hierarchical clustering was performed for individual technical replicates. ( b ) Competitive inhibition of EV interactions with lectin microarray with mannose (Man) and N -acetylglucosamine (GlcNAc). ( c ) Lectin microarray of PNGase F-treated EVs. Significance (* = p ≤ 0.05) was determined by the ANOVA test.

Article Snippet: Probes (neoglyconjugates, glycoproteins and lectins) were printed on Nexterion® Slide H (Schott, Mainz, Germany) using a SciFlexArrayer S3 piezoelectric microarray printer (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C essentially as previously described .

Techniques: Inhibition, Microarray

Journal: bioRxiv

Article Title: A Photonic Biosensor-Integrated Tissue Chip Platform for Real-Time Sensing of Lung Epithelial Inflammatory Markers

doi: 10.1101/2022.06.21.497028

Figure Lengend Snippet: a) Design in GDS (Graphic Design System) format used to produce photolithographic masks for the photonic chip. The overall footprint is 5.8 × 4.0 mm, with 2 banks of 7 × 2 ring resonators, each with a total diameter of 300 μm including the trench (open area in the top layer of SiO 2 allowing direct contact between the ring resonator and analyte-containing solution). One temperature control ring (under oxide) is also provided in each bank. b) A photograph of the chip after fabrication and dicing. c) Functionalization chemistry using a succinic anhydride-bearing silane. d) Photomicrograph of a ring bank, with the antibody layout superimposed. e) An image acquired by the microarrayer immediately after spotting shows reproducible spotting of antibody solutions and StabilGuard over the rings. The dark spots are ∼250 pL drops settled in the ring trenches.

Article Snippet: After silanization, antibody solutions were printed on the rings using a Scienion SX SciFLEXarrayer piezoelectric microarrayer equipped with a PDC-60 capillary nozzle.

Techniques: Control

Journal: Cells

Article Title: Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation

doi: 10.3390/cells12030351

Figure Lengend Snippet: Results of univariate Cox regression analysis after sorting patients based on their primary disease in correlation with data obtained from lectin microarray with GNL between survivors ( n = 39) and non-survivors ( n = 13).

Article Snippet: Briefly, fibrinogen samples were printed on epoxysilane-coated microarray slides (NEXTERION Slide E, Schott, Germany) in eight identical subarrays using piezoelectric printer sciFLEXARRAYER S1 equipped with piezo dispense capillary PDC 80 (Scienion AG, Berlin, Germany), at a temperature of 14 °C and humidity of 60%.

Techniques: Microarray